FIG. 9.

Histone modifications in primary cells. (A) ChIP analyses were performed utilizing the same primer-probe combinations as in Fig. 3. The abbreviations are as defined in the legend to Fig. 3. Hematopoietic stem cells (HSC) were examined untreated. Primary monocytes were examined fresh as well as 30 min and 90 min after 1-μg/ml LPS stimulation. These results represent an average of duplicates or triplicates and at least two separate experiments. (B) H3 lysine 9 trimethylation, a repressive mark, was examined in hematopoietic stem cells and peripheral blood monocytes. The NTERA-2cl.D2 cell line was used as a positive control.