FIG. 7.

E2-dependent regulation of constitutively bound Pol II at target promoters. ChIP-qPCR was used to determine the occupancy by ERα, Pol II, Ser2-phosphorylated Pol II, and NELFA at the TFF1 (A) and CYP1B1 (B) promoters. The empty and filled/hatched bars represent ChIP enrichment for untreated (-U) and E2-treated (-E) cells, respectively. “−” and “+” indicate the regions upstream and downstream of the TSS, respectively. Each bar represents the mean + SEM for at least three separate determinations.