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Activities of compartment-specific enzymes in E. coli CFT073 cell fractions
| Enzyme | Mean sp act (U/mg) ± SDa
|
||
|---|---|---|---|
| Cytoplasm | Whole membraneb | Outer membrane | |
| Esterase | 0.4 ± 0.2 | 5.5 ± 0.1 | 16.5 ± 6.6* |
| NADH oxidase | 0.1 ± 0.2 | 23.4 ± 3.9 | 2.0 ± 3.5* |
| Glucose-6-phosphate dehydrogenase | 1.7 ± 0.8 | ND | ND |
ND, no detectable activity; *, P < 0.05 by a Student's t test (compared to activity in other fractions).
Contains inner and outer membrane fractions.
