TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5 | F−recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 | 33 |
| S17-1λpir | recA thi pro hsdR M+ Smr <RP4:2-Tc:Mu:Ku:Tn7> Tpr | 86 |
| Y. pseudotuberculosis | ||
| YPIII/pIB102 | yadA::Tn5 Kmr (wild type) | 8 |
| YPIII07/pIB102 | YPIII, cpxA in-frame deletion of codons 41 to 449, Kmr | This study |
| YPIII08/pIB102 | YPIII, cpxR in-frame deletion of codons 11 to 193, Kmr | This study |
| YPIII09/pIB102 | YPIII, rpoE in-frame deletion of codons 3 to 181, Kmr | This study |
| YPIII40/pIB102 | YPIII, baeS in-frame deletion of codons 24 to 437, Kmr | This study |
| YPIII29/pIB102 | YPIII, baeR in-frame deletion of codons 22 to 227, Kmr | This study |
| YPIII28/pIB102 | YPIII, rseA in-frame deletion of codons 14 to 194, Kmr | This study |
| YPIII42/pIB102 | YPIII, rseB in-frame deletion of codons 22 to 290, Kmr | This study |
| YPIII/pIB26 | pIB102, exchange of lcrQ with a streptomycin-spectinomycin resistance cassette, Kmr Smr Spr | 76 |
| YPIII07/pIB26 | YPIII/pIB26, cpxA in-frame deletion of codons 41 to 449, Kmr Smr | This study |
| YPIII/pIB202 | pIB102, yscF in-frame deletion, Kmr | 23 |
| Plasmids | ||
| pCR4-TOPO | TA cloning vector, Kmr Cbr | Invitrogen |
| pDM4 | Suicide plasmid carrying sacBR, Cmr | 58 |
| pPJE006 | ∼925-bp XhoI/XbaI PCR fragment of ΔcpxA(41-449) in pDM4, Cmr | This study |
| pMF586 | ∼485-bp XhoI/XbaI PCR fragment of ΔcpxR(11-193) in pDM4, Cmr | This study |
| pMF587 | ∼510-bp XhoI/XbaI PCR fragment of ΔrpoE(3-181) in pDM4, Cmr | This study |
| pMF682 | ∼410-bp XhoI/XbaI PCR fragment of ΔbaeS(24-437) in pDM4, Cmr | This study |
| pKEC009 | ∼445-bp XhoI/XbaI PCR fragment of ΔbaeR(22-227) n pDM4, Cmr | This study |
| pKEC010 | ∼420-bp XhoI/XbaI PCR fragment of ΔrseA(14-194) in pDM4, Cmr | This study |
| pMF680 | ∼460-bp XhoI/XbaI PCR fragment of ΔrseB(22-290) in pDM4, Cmr | This study |
| pJF006 | ∼1,020-bp XhoI/XbaI PCR fragment of cpxR encoding the mutation D51A in pDM4, Cmr | This study |
| pMMB208 | Expression vector, Cmr | 62 |
| pKEC021 | 700-bp XbaI/KpnI PCR fragment of cpxR in pMMB208, Cmr | This study |
| pJF015 | 700-bp XbaI/KpnI PCR fragment of cpxR encoding the mutation D51A in pMMB208, Cmr | This study |
| pET22b(+) | Expression vector, Ampr | Novagen |
| pMF581 | 1,381-bp NdeI/EagI PCR fragment of cpxA in pET22b(+), Cbr | This study |