FIG. 1.
Growth inhibition of a feoB mutant by a hyperpigmented mutant of L. pneumophila. Approximately 5 × 105 CFU of either an feoB mutant (A) or wild-type 130b (B) were spread onto BCYE agar lacking its standard ferric iron supplement and then aliquots of BYE medium containing either 5 × 103 CFU of wild-type 130b (top), hmgA mutant NU326 (left), or complemented NU326 (phmgA) (bottom) or no added bacteria (right) were spotted onto the plates. After 5 days of incubation at 37°C, the growth of the feoB mutant or strain 130b around the spots was recorded. The results are representative of at least three replicate experiments.