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. 2007 May 21;75(8):3859–3867. doi: 10.1128/IAI.00159-07

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FIG. 3. — Secretion of FspA. (A) Detection of FspA_81-176 in whole cells or supernatants. WT, wild-type 81-176; M, 81-176 fspA_81-176::cat (strain PG2572); C, 81-176 fspA_81-176::cat (pCPE2575) (strain PG2573); WC, whole cells; S, supernatants. (B) Controls for bacterial cell leakage. Whole cells and supernatants of wild-type 81-176 were detected with antibodies against recombinant forms of Cj0977, Omp18, and Omp50 (4, 5, 13). WC, whole cells; S, supernatants. (C) Detection of FspA₈₄₈₆ in wild-type CG8486 and strain PG2662 (wild-type 81-176 containing FspA₈₄₈₆ inserted into astA) and PG2663 (81-176 fspA_81-176::cat containing FspA₈₄₈₆ inserted into astA). (D) Secretion of FspA_81-176 campylobacter strains with and without plasmid pCPE2575 carrying fspA_81-176. TGH, C. jejuni strain TGH9011.