FIG. 3.

TNF production by BMDM infected at different MOIs with the mycolactone A/B-producing strain 98-912 or the mycolactone-negative strain 5114. BMDM were infected at MOIs ranging from 10:1 to 1:15 with the M. ulcerans strains 5114 (A) or 98-912 (B). Twenty-four hours postinfection, the supernatant from three independent wells was removed for each strain and stored frozen until cytokine measurement by ELISA. BMDM infected with M. ulcerans 5114 produce high amounts of TNF at high MOIs, and TNF production decreases as the MOI decreases. With M. ulcerans 98-912, production of TNF is low, independent of the MOI. Results are from one representative experiment out of three independent experiments.