TcdB-induced hexosaminidase release does not correlate with cell death. The percentages of apoptotic cells among control cells and among cells treated for 5 h with TcdB (6 nM), latrunculin B (Lat Β, 2 μg/ml), staurosporin (Stau, 1 mg/ml), or PMA (100 nM)-ionophore A23187 (10 μM) were estimated by FACS analysis (upper panel). The corresponding hexosaminidase release is shown in the lower panel.