. 2007 May 21;75(8):3758–3768. doi: 10.1128/IAI.00225-07

TABLE 1.

Phenotypic analysis of Py52/Py36-deficient sporozoites and LSs

Parasite population	No. of salivary gland sporozoites per mosquito^a	Hepatocyte entry (no. of cells)^b		No. of LSs at 43 h^c
Parasite population	No. of salivary gland sporozoites per mosquito^a	Outside	Inside	No. of LSs at 43 h^c
P. yoelii wt clone	30,389 ± 13,241	593 ± 58.63	502 ± 61.82	278.25 ± 79.88
Py52/Py36-deficient Cl1	24,031 ± 11,834	849.66 ± 136.64	272 ± 54.5	1 ± 1
Py52/Py36-deficient Cl2	21,822 ± 7,639	842.33 ± 75.79	284 ± 79	1 ± 1.41
P. yoelii wt clone + CytD^d	NA	1,046.66 ± 47.89	27 ± 9.53	ND

The mean number of sporozoites was determined from at least three independent mosquito feeding experiments at day 14 after the infectious blood meal. NA, not applicable.

For this specific test, hepatocyte entry is defined as the sporozoite's ability to cross a host cell membrane and to enter the cell. Sporozoites were counted in at least three different wells. Eight fields per well were examined.

Experiments were done in duplicate. ND, not determined.

As a negative control, wt sporozoite motility was inhibited by treatment with 1 μM cytochalasin D (CytD) for 15 min at 37°C.