Table 1.
Identification of yeast U1 snRNP proteins
Mr (app.) (kDa) | Mr (calc.) (kDa) | Peptides sequenced | Protein name | Corresponding gene | Accession number |
---|---|---|---|---|---|
69 | 75.1 | 7 | Prp39p | PRP39 | L29224L29224 |
69 | 69.1 | 3 | Prp40p | PRP40 | P33203P33203 |
37 | 34.4 | 3 | Mud1p | MUD1 | P32605P32605 |
34 | 34.4 | 2 | Snp1p | SNP1 | X59986X59986 |
31 | 27.0 | 3 | U1-C | SCL8003 17 | U17243U17243 |
18 | 16.3 | 3 | Sm D1 | SMD1 | Q02260Q02260 |
15 | 12.1 | 5 | Sm D2 | SCL9328 3 | U17245U17245 |
12 | 10.4 | 1* | Sm E | SC55020 15 | U55020U55020 |
11 | 9.6 | 2 | Sm F | SCL9705 11 | U25842U25842 |
10 | 11.2 | 4 | Sm D3 | SMD3 | P43321P43321 |
9 | 8.5 | 2 | Sm G | SNP2 | P40204P40204 |
The identity of this peptide was further ascertained by methylation and 18O-labeling.