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. 2007 May 25;75(8):3989–3998. doi: 10.1128/IAI.00388-07

FIG. 2.

FIG. 2.

Sequential stimulation of PMN by high doses (10−5 M) of fMLP, followed 5 min later by 10−7 M fMLP. (a) Control; (b) 5 mM EGTA added 15 s before the first fMLP injection; (e) control and oxidative product release (see Materials and Methods); (f) 10−5 M fMLP followed by 120 μg/ml IC. (c and d) Reversal of the order of sequential stimulation, 10−7 M fMLP, followed by 10−5 M fMLP 5 min later. (c) Control; (d) 5 mM EGTA added 15 s before the first fMLP injection. Each figure is representative of four independent experiments.