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Sequential stimulation of PMN by saturating doses (10−7 M) of fMLP followed by 120 μg/ml IC 5 min later. (a) Control; (b) 5 mM EGTA added 15 s before fMLP injection; (c) 5 mM EGTA added before IC injection; (d) release of elastase (shown as F460) and of oxidative products (shown as F530). Each figure is representative of five independent experiments.
