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. 2007 May 25;75(8):3989–3998. doi: 10.1128/IAI.00388-07

FIG. 4.

FIG. 4.

Sequential stimulation of PMN by saturating doses (120 μg/ml) of IC followed 5 min later by 10−7 M fMLP. (a) Control; (b) 5 mM EGTA added 15 s before IC injection; (c) 5 mM EGTA added before fMLP injection; (d) release of elastase (shown as F460) and of oxidative products (shown as F530). Each figure is representative of five independent experiments.