TABLE 1.
Primers and probes used for detection of porcine 28S rRNA and TNF-α, IL-8, and IL-18 mRNAs by real-time RT-PCR
| Primer or probe | Sequence 5′-3′a |
|---|---|
| 28S rRNA.fwd | GCTCCACGGGAGGTTTCTG |
| 28S rRNA.rev | GGTACACCTGTCAAACGGTAACG |
| 28S rRNA.probe | (FAM)-CTCCCTGAGCTCGCCTTAGGACACCT-(TAMRA) |
| TNF-α.fwd | AAGGACTCAGATCATCGTCTCAAAC |
| TNF-α.rev | CGGCTTTGACATTGGCTACA |
| TNF-α.probe | (FAM)-CGTGGGCGACGGGCTTATCTGA-(TAMRA) |
| IL-8.fwd | AGTTTTCCTGCTTTCTGCAGCT |
| IL-8.rev | TGGCATCGAAGTTCTGCACT |
| IL-8.probe | (FAM)-ACTCTTGCCAGAACTGCAGCCTCACA-(TAMRA) |
| IL-18.fwd | TCCTTTTCATTAACCAGGGACATC |
| IL-18.rev | GGTCTGAGGTGCATTATCTGAACA |
| IL-18.probe | (FAM)-CAGAATCAGGCATATCCTCAAACACGGCT-(TAMRA) |
a
FAM, 6-carboxyfluoroscein; TAMRA, 6-carboxytetramethylrhodamine.