TABLE 2.
Primers and probes used for detection of mgtC, sipC, sseC, and yejA mRNAs by real-time RT-PCR
| Primer or probe | Sequence 5′-3′a |
|---|---|
| sipC.fwd | GGACGAAGCCCGTGAAAGT |
| sipC.rev | TGCTCTCCATTGTTTTCAGCATT |
| sipC.probe | (FAM)-CCTGAATCAGGCTGGTCGATTTACG TG-(TAMRA) |
| sseC.fwd | GCAGGTTGTGCAGGAATGGT |
| sseC.rev | TGGTCAGCACCGCACATC |
| sseC.probe | (FAM)-TGCCGTTTCGGCTCCGGCT-(TAMRA) |
| yejA.fwd | TCAGCGATCCGCTTTCAAC |
| yejA.rev | GCCCATTTTCCACTGAGTAATCC |
| yejA.probe | (FAM)-CCGCCCTTAGCCAGCGGGCCA AT-(TAMRA) |
a
FAM, 6-carboxyfluoroscein; TAMRA, 6-carboxytetramethylrhodamine.