TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant description | Source or reference |
|---|---|---|
| Strains | ||
| P. multocida | ||
| X73 | Serotype A:1 | 14 |
| VP161 | Serotype A:1, Vietnamese isolate from chickens | 35 |
| AL435 | VP161 carrying a Tn916 insertion in gene PM1417, fully virulent | This study |
| AL838 | VP161 containing pAL99 | This study |
| AL721 | kdkA mutant of AL435 | This study |
| AL839 | AL721 containing pAL99 | This study |
| AL840 | AL721 containing pAL462 | This study |
| AL690 | hptB mutant of AL435 | This study |
| AL844 | AL690 containing pAL99 | This study |
| AL845 | AL690 containing pAL464 | This study |
| AL836 | Nonpolar hptA mutant of AL435 | This study |
| AL846 | AL836 containing pAL99 | This study |
| AL847 | AL836 containing pAL465 | This study |
| E. coli | ||
| DH5α | deoR endA1 gyrA96 hsdR17 (rK− mK+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argFV169) φ80lacZΔM15 F− | Bethesda Research Laboratories |
| Sm10 λ pir | Strain for propagation of pUA826 and its derivatives | 23 |
| Plasmids | ||
| pUA826 | Mob+, R6K replicon, Apr Strr Spcr, single-crossover insertional mutagenesis vector | 7 |
| pUA826tpi | As for pUA826 but containing 240-bp PstI fragment containing P. multocida tpiA promoter region, nonpolar mutagenesis vector | This study |
| pAL99 | P. multocida expression plasmid (Kanr), constitutive tpiA promoter upstream of BamHI | 13 |
| pAL462 | Complete kdkA gene cloned into pAL99 | This study |
| pAL464 | Complete hptB gene cloned into pAL99 | This study |
| pAL465 | Complete hptA gene cloned into pAL99 | This study |