TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Sequence	Description
BAP3813	TTTGGAGTCGACAAGCGCGTATTCTTGGTT	kdkA internal forward primer for mutagenesis
BAP3814	CACTTTGTCGACGTTCACCAAGCTGATGTG	kdkA internal reverse primer for mutagenesis
BAP3914	AAAGGGATCCGTGGCCATTCGCTATCTCTG	kdkA flanking forward primer for expression
BAP3915	TATGTGTCGACCTTATGCTTGATATCCCGC	kdkA flanking reverse primer for expression
BAP3721	TATGTTGTCGACAAGTTGATTGGGTGGTCGAAG	hptB internal forward primer for mutagenesis
BAP3722	TAAATGGTCGACTCCGGTGTCAACAGAAACAA	hptB internal reverse primer for mutagenesis
BAP3745	TAATTGGGATCCAACCGGAATTGGTATTCG	hptB flanking forward primer for expression
BAP3746	TCAAGAGTCGACTGCCGTATAGGCAGTTTCAA	hptB flanking reverse primer for expression
BAP3719	CAAAACGTCGACGCAGTTACTGGCTGGTAT	hptA internal forward primer for mutagenesis
BAP3720	TCAGTAGTCGACAACTTACATTGGGCGAGGA	hptA internal reverse primer for mutagenesis
BAP3966	CACACAGGATCCATTGGACGAGATAATGATGCCG	hptA flanking forward primer for expression
BAP3967	ATAACAGTCGACGGTTCATCATTGAACGTC	hptA flanking reverse primer for expression
BAP2782	GCCCTACACAAATTGGGAGA	pUA826 primer near SalI site