TABLE 3.
Negative-ion CE-ES-MS data and proposed compositions of LPS-OH from the P. multocida VP161 wild-type and mutant strainsa
| Strain (relevant description) | Observed ions (m/z)
|
Molecular ion (m/z)
|
Relative intensity | Proposed composition | ||||
|---|---|---|---|---|---|---|---|---|
| (M − 4H)4− | (M − 3H)3− | (M − 2H)2− | Observed | Calculated | ||||
| VP161 (wild type) | 992.4 | 2,980.2 | 2,977.6 | 0.1 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH | |||
| 999.6 | 3,001.8 | 2,999.5 | 0.3 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH | ||||
| 1,033.0 | 3,101.0 | 3,100.7 | 0.3 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn | ||||
| 1,040.7 | 3,125.1 | 3,122.5 | 0.7 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH-PEtn | ||||
| 1,081.7 | 3,247.7 | 3,245.6 | 1.0 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH-PEtn | ||||
| AL690 (hptB) | 504.4 | 757.2 | 1,516.1 | 1,515.4 | 0.2 | 2Kdo, lipid A-OH-PEtn | ||
| 780.3 | 1,040.4 | 3,124.7 | 3,122.5 | 0.2 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH-PEtn | |||
| 810.8 | 1,081.6 | 3,247.7 | 3,245.6 | 1.0 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH-PEtn | |||
| AL844 (hptB + vector) | 757.5 | 1,516.1 | 1,515.4 | 0.2 | 2Kdo, lipid A-OH-PEtn | |||
| 1,040.8 | 3,124.7 | 3,122.5 | 0.3 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH-PEtn | ||||
| 1,081.7 | 1,622.7 | 3,247.7 | 3,245.6 | 1.0 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH-PEtn | |||
| AL845 (hptB, complemented) | 1,033.0 | 3,101.0 | 3,100.7 | 0.2 | 2PCho, 3Hex, 4Hep, 2Kdo, Lipid A-OH-PEtn | |||
| 1,040.7 | 1,561.2 | 3,124.7 | 3,122.5 | 0.3 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH-PEtn | |||
| 1,081.8 | 1,622.8 | 3,247.7 | 3,245.6 | 1.0 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH-PEtn | |||
| AL836 (hptA) | 616.8 | 1,235.6 | 1,252.2 | 0.1 | Kdo-P, lipid A-OH (− H2O) | |||
| 451.6 | 678.4 | 1,358.8 | 1,375.2 | 0.5 | Kdo-P-PEtn, lipid A-OH (− H2O) | |||
| 1,033.4 | 1,549.9 | 3,102.6 | 3,100.6 | 1.0 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn | |||
| AL846 (hptA + vector) | 616.8 | 1,235.6 | 1,252.2 | 0.1 | Kdo-P, lipid A-OH (− H2O) | |||
| 451.6 | 678.3 | 1,358.8 | 1,375.2 | 0.5 | Kdo-P-PEtn, lipid A-OH (− H2O) | |||
| 774.7 | 1,033.2 | 1,550.3 | 3,102.6 | 3,100.6 | 1.0 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn | ||
| AL847 (hptA, complemented) | 1,033.3 | 3,101.0 | 3,100.7 | 0.5 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn | |||
| 1,040.7 | 1,561.2 | 3,124.7 | 3,122.5 | 1.0 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH-PEtn | |||
| 1,081.5 | 1,622.4 | 3,247.7 | 3,245.6 | 0.9 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH-PEtn | |||
| AL721 (kdkA) | 743.8 | 992.3 | 2,979.9 | 2,977.6 | 0.2 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH | ||
| 775.0 | 1,033.1 | 3,103.1 | 3,100.6 | 1.0 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn | |||
| 825.5 | 1,101.2 | 3,306.3 | 3,303.6 | 0.2 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn-P-PEtn | |||
| AL839 (kdkA + vector) | 1,033.1 | 1,550.3 | 3,103.1 | 3,100.6 | 1.0 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn | ||
| AL840 (kdkA, complemented) | 1,033.3 | 3,101.0 | 3,100.7 | 0.2 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH-PEtn | |||
| 1,040.7 | 1,561.3 | 3,124.7 | 3,122.5 | 0.5 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH-PEtn | |||
| 1,081.7 | 1,622.8 | 3,247.7 | 3,245.6 | 1.0 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH-PEtn | |||
a
Average mass units were used for calculation of molecular weights based on the following proposed composition: lipid A, 952.00; Hex, 162.15; Hep, 192.17; Kdo, 220.18; Kdo-P, 300.13; PEtn, 123.05; PCho, 165.05.