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. 2007 May 25;75(8):3812–3822. doi: 10.1128/IAI.00341-07

FIG. 3.

FIG. 3.

Effects of intranasal immunization of baboons with the polylysine-linked synthetic peptide vaccine (400, 800, and 1,600 μg), the recombinant LC3 protein vaccine (200 μg), or CT adjuvant (20 μg) alone as assessed by an ELISA using immunoaffinity chromatography-purified galactose-inhibitable lectin as the capture antigen. The synthetic peptide vaccine did not elicit significant serum antilectin IgA antibodies (A) as determined by the ELISA (P > 0.11). There was a statistically significant but meager serum antilectin IgG antibody response (B) at the 1,600-μg dose (P, 0.003 over 4 weeks and 0.0004 at 4 weeks compared to the control). Of greatest interest, the synthetic peptide vaccine at the 400- and 1,600-μg doses elicited an intestinal antilectin IgA response (C) compared to the control after 4 weeks (P = 0.004). There was a positive dose-dependent response (P = 0.0002) and an increase in the ELISA OD reading over time (P = 0.002). The LC3 vaccine elicited substantial serum antilectin IgA (P = 0.02) (A) and IgG (P < 0.0002) (B) antibody responses over time and at 4 weeks. In contrast, the LC3 vaccine did not induce any intestinal antilectin IgA response (C) by 28 days (P = 0.80).