TABLE 1.
qRT-PCR gene-specific primers
| Gene | Rd gene no. | Full name | Primer sequences | Taa (°C) | Product length (bp) |
|---|---|---|---|---|---|
| gyrA | HI1264 | DNA gyrase subunit A | 5′-ACTGCTGGCGGTATTGTG-3′, 5′-CGCGACCTTGTGATGAGAAC-3′ | 63 | 149 |
| hemR | HI0113 | Hemin receptor | 5′-GTGACGCTGAGGATGTTAGAGTG-3′, 5′-GCCACCATTACCATATTGAGGAG-3′ | 63 | 149 |
| frdB | HI0834 | Fumarate dehydrogenase subunit B | 5′-GTAACGAAGCACCAGCATTAG-3′, 5′-ACCACAGTTGATACACATTGAG-3′ | 60 | 125 |
| recA | HI0600 | Recombinase A | 5′-GGATTGGTGGTTTGCCTATG-3′, 5′-AATGACGGAAAGAGTTAATGTTG-3′ | 59 | 86 |
a
Ta, optimum annealing temperature, determined experimentally via temperature gradient.