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Southern blot analysis for the adenine deaminase gene (ade) in B. hermsii. (A) Agarose gel with genomic DNA digested with EcoRI and stained with ethidium bromide. (B) Hybridization with the probe containing the sequence present in both the intact gene and pseudogene. (C) Hybridization with the probe with the sequence present only in the intact gene. Isolate designations are indicated above each lane. Molecular size standards are shown on the left.
