TABLE 4.
Primer and fluorescent probe sequences for real-time RT-PCR
| Gene | Primers and probe | Sequence (5′ to 3′) |
|---|---|---|
| hpt | Forward | AAGAATTAGCCCAAAAGATTAGAAACTACT |
| Probe | CACTTCTTAAAGGCTCTTTCATGTTT | |
| Reverse | CGTTTAATCCAATCTCTCTTGTAATATCTG | |
| purA | Forward | TCAAGAGTTGGTTCAGGACCTTTT |
| Probe | TCTTATCTCCAATAGGTTCTAGGATTTCAGTT | |
| Reverse | GCCATATTCCTGCCCCTTTT | |
| purB | Forward | GGCGGATTTATAGCTGCAACTC |
| Probe | TGCAAAAGACTAAACATTCCTCAAGCAT | |
| Reverse | TCAGGGCAGCATCAGTAGCTAAG | |
| flaB | Forward | AAGTCAGCTGCTCAAAATGTAAAAAC |
| Probe | TTTGCGGGTTGCATTCCAAGCTCTT | |
| Reverse | CAGCTAGTGATGCTGGTGTGTTAAT |