TABLE 5.
Transcription of B. hermsii purine salvage genes in vitro and in vivo
| Gene | Transcript amounts of hpt, purA and purB (ng, mean ± SD) compared to flaBa
|
Ratio (34°C/24°C) | ||
|---|---|---|---|---|
| 34°C culture | 24°C culture | In vivo | ||
| hpt | 12.54 ± 7.04 | 7.43 ± 2.64 | 2.64 ± 0.72 | 1.69 |
| purA | 4.88 ± 0.16 | 2.74 ± 0.20 | 0.53 ± 0.21 | 1.78 |
| purB | 3.57 ± 0.31 | 2.54 ± 1.13 | 1.38 ± 0.39 | 1.41 |
a
Relative amounts of mRNA from B. hermsii DAH grown at two culture temperatures or isolated from mouse blood were determined by real-time RT-PCR with flaB as the standard. flaB transcripts were normalized to 49 ng in each assay for comparison with the other genes.