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. 2007 Jun 8;189(16):5937–5946. doi: 10.1128/JB.00428-07

TABLE 1.

Kinetic parameters of recombinant SfcA and MaeB

Parameter Valuea
SfcA MaeB
Malate oxidative decarboxylation (pH 7.5)
    kcat,NAD (s−1) 82.7 ± 5.1 ND
    Km,NAD (μM) 68.8 ± 3.2 ND
    kcat,NAD/Km,NAD 1.2
    kcat,NADP (s−1) 12.4 ± 0.6 66.6 ± 2.5
    Km,NADP (μM) 1,000 ± 78b 41.5 ± 1.5
    kcat,NADP/Km,NADP 0.012 1.6
    Km,malate (mM) 0.66 ± 0.04 3.41 ± 0.25b
    Km,Mg (mM) 2.03 ± 0.1 0.19 ± 0.01
Pyruvate reductive carboxylation (pH 7.0)
    kcat (s−1) 2.93 ± 0.15 0.38 ± 0.02
    Km,pyruvate (mM) 2.59 ± 0.1 6.21 ± 0.4
    kcat,forward/kcat,reverse 28.2 175.2
a

Kinetic values are given as averages ± standard deviations. Each value was averaged over at least two different enzyme preparations. ND, no activity was detected.

b

Kinetics for these reactions are sigmoidal, and the reported values are K0.5 values, not true Km values. The calculated Hill coefficients were as follows: 1.95 for K0.5,NADP of SfcA and 1.42 for K0.5,malate of MaeB.

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