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Plg overlay assay carried out with the cell wall fraction of B. lactis BI07 resolved in a two-dimensional gel stained for protein (A) and in a replicate two-dimensional gel used for Plg overlay (B). The squares indicate the major Plg-binding proteins; 4 μg/ml of Plg was applied in the Plg overlay. Plg-binding proteins were detected with anti-Plg antibody and peroxidase-conjugated second antibody. Table1 shows the identities of the putative Plg-binding proteins.
