Strains |
|
|
E. coli
|
|
|
S17.1 |
RP4-2 Tc::Mu Km::Tn7
|
50 |
BL21(DE3) |
Host for overexpression of GlnBHis6 and GlnKHis6
|
Novagen |
R. capsulatus
|
|
|
B10S |
Wild-type Smr
|
32 |
BSRUB13 |
ΔglnK
|
This work |
PHU332 |
glnB::Kmr
|
P. Hübner, Basel, Switzerland |
RCAY22 |
amtY::Gmr
|
62 |
RCAY63 |
amtB::Kmr
|
62 |
SG21 |
glnK::Gmr
|
This work |
TR3 |
glnB51 orf3528::Gmr
|
This work |
Plasmids |
|
|
pAP2 |
pJB3TC20 derivative carrying glnK51 (GlnK-Y51F) and 300 bp of upstream sequence |
This work |
pAY98 |
pMECA derivative carrying glnKamtB; Apr
|
A. Yakunin |
pBSRUB13 |
pNIRUB145 derivative carrying lacZc expressed from a Km cassette; Gmr
|
This work |
pBSRUB19 |
pPHU231 derivative carrying amtB and most of glnK
|
This work |
pBSRUB21-I |
pBSRUB19 derivative carrying amtB expressed from a Km cassette |
This work |
pET-22b(+) |
T7 expression vector; Apr
|
Novagen |
pJB3TC20 |
Broad-host-range vector; mob Apr Tcr
|
5 |
pK18 |
Cloning vector; Kmr
|
46 |
pML5 |
Broad-host-range lacZ-fusion vector; mob Tcr
|
35 |
pNIRUB60-I |
pUC18 derivative carrying aphII (Kmr) of transposon Tn5
|
This work |
pNIRUB142 |
pML5 derivative carrying lacZc expressed from a Km cassette |
This work |
pNIRUB143 |
pSUP202 derivative carrying most of amtB
|
This work |
pNIRUB144 |
pNIRUB143 derivative carrying glnK::GmramtB
|
This work |
pNIRUB145 |
pNIRUB144 derivative with 228-bp deletion in glnK
|
This work |
pPHU231 |
Broad-host-range vector; mob Tcr
|
47 |
pSG5IIa |
pUC18 derivative carrying glnK::Gmr; Apr
|
This work |
pSG18 |
pK18 derivative carrying most of amtB
|
This work |
pSGNJ1 |
pSVB10 derivative carrying glnK-amtB; Apr
|
This work |
pSUP202 |
Mobilizable cloning vector; mob Tcr Apr Cmr
|
50 |
pSV5 |
pET-22b(+) derivative carrying glnBHis6
|
This work |
pSV6 |
pET-22b(+) derivative carrying glnKHis6
|
This work |
pSVB10 |
Cloning vector; Apr
|
2 |
pTR9 |
pK18 derivative carrying 5′ part of glnB and orf3528
|
This work |
pTR10 |
pUC18 derivative carrying 3′ part of glnB
|
This work |
pTR11 |
pTR9 derivative carrying Gmr from pWKR440 inserted into orf3528
|
This work |
pTR12 |
pTR10 derivative; mob Tcr
|
This work |
pTR14-I |
pTR12 derivative carrying glnB51 (GlnB-Y51F) and orf3528::Gmr
|
This work |
pUC18 |
Cloning vector; Apr
|
55 |
pWKR56-I |
Mobilizable cloning vector; mob Tcr
|
32 |
pWKR440 |
pACYC derivative carrying Gmr Tcr
|
11 |