TABLE 2.
[14C]Acetate labeling of strains deficient in PlsX, PlsY, or PlsCa
| Strain | Total lipid count/min/108 cells (%) | Distribution (%)
|
|||
|---|---|---|---|---|---|
| PL | MAG | DAG | FA | ||
| LP39(Pxyl-plsX) + xylose | 15,782 | 89.5 | — | 9.3 | 0.2 |
| LP39(Pxyl-plsX) without xylose | 1,679 (10.6) | 89.4 | — | 9.6 | — |
| LP61(Pspac-plsY) + IPTG | 9,603 | 86.2 | — | 11.8 | 2.0 |
| LP61(Pspac-plsY) without IPTG | 8,011 (83.4) | 31.8 | — | 0.5 | 63.2 |
| LP15(Pspac-plsC) + IPTG | 12,938 | 87.5 | — | 12.5 | — |
| LP15(Pspac-plsC) without IPTG | 32,179 (249) | 8.0 | 6.2 | 0.5 | 85.3 |
a
Total lipid is given as counts per min per 108 cells. The strains were grown as described in the text with or without IPTG and labeled with [14C]acetate for 30 min. The lipids were extracted as described in Materials and Methods. The distribution of lipids was determined by thin-layer chromatography using the neutral lipid solvent system shown in Fig. 3, and the percentage of each indicated lipid class was determined by quantifying the PhosphorImager data. PL, phospholipid; MAG, monoacylglycerol; DAG, diacylglycerol; FA, fatty acid; —, not detected. The data shown are representative of three independent experiments carried out in both laboratories.