FIG. 4.

After heat shock TraM steady-state levels are transiently reduced dependent on a functional GroEL protein. Western blot analyses of TraM and GroEL steady-state levels in E. coli MC4100(R1-16) (wild type [wt]) and NRK117(R1-16) [groEL(Ts)] were performed with (A) and without (C) heat shock. (B and D) Quantification of TraM and GroEL signals. The intensity of the TraM or the GroEL signals in wild-type cells 90 min after heat shock or dilution was set as 1. Data points that are not depicted indicate that no protein was detectable at these time points. St, stationary phase at 22°C/37°C.