FIG. 3.
Influence of RovA on the expression of rovA. To determine the effect of RovA on the rovAYent (A) and rovAYpstb/Ypestis (B) promoters, expression patterns were determined in wild-type Y. enterocolitica (filled symbols) and a ΔrovA mutant (open symbols). Four GFP reporters were utilized: Y. enterocolitica rovA reporter fusions (squares, pYEL; triangles, pYES) and Y. pseudotuberculosis/Y. pestis rovA reporter fusions (circles, pYPL; diamonds, pYPS). Gray circles show the results for vector-only controls. The data are expressed in relative light units (R.L.U.) as a function of the OD600. (C) Expression of pYPL and pYEL in wild-type Y. pestis (filled bars) and a ΔrovA mutant (open bars). The data are expressed in relative light units (R.L.U.)/OD600. Experiments were performed in triplicate, and error bars were plotted but are typically too small to see clearly.