Influence of H-NS on the expression of rovA. To determine the effect of H-NS on the rovAYpstb/Ypestis (A) and rovAYent (B) promoters, expression patterns were determined in E. coli MC4100 (WT), an hns mutant [hns(−)], or an hns mutant complemented with Y. enterocolitica hns [hns(−) pHNS]. Filled bars, pYPL (A) or pYEL (B); open bars, pYPS (A) or pYES (B); gray bars, vector control. (C) Expression of pYPL (diamonds) and pYEL (squares) in wild-type Y. enterocolitica (filled symbols) and pHNS-complemented Y. enterocolitica (open symbols). The data are expressed in relative light units (R.L.U.) as a function of the OD600.