FIG. 7.

LeuO regulation of rovA in Y. enterocolitica. (A) To determine the effect of LeuO on rovA transcription in Y. enterocolitica, the levels of β-galactosidase activity of the rovA::lacZ reporter were compared in the wild type (WT), a transposon mutant with a mutation in the promoter of leuO (TM102), a mutant with an in-frame deletion of leuO (ΔleuO), and a strain containing leuO on a multicopy plasmid (pLEUO). The expression is shown as the fold change compared with wild-type expression. (B) Whole-cell proteins (equivalent to 0.1 OD₆₀₀ unit) from wild-type (WT) and TM102 cultures were harvested and analyzed by Western blotting with anti-H-NS antibody. Purified His-tagged H-NS (rH-NS) served as a positive control.