TABLE 2.
Primers used in this study
| Primer | Sequencea |
|---|---|
| Deletion constructs | |
| lacZ | |
| lacZ5′F | ACGCGTCGACGCCAGCAATACCCCATTTAGC |
| lacZ5′R | CGGGATCCAATTTCAGCCTTATCTTTTACGAAAGTTAGC |
| lacZ3′F | CGGGATCCGCAACAATATCAACACAGAATTTCTAATACGC |
| lacZ3′R | ATAAGAATGCGGCCGCTGCTGGGCTATAATCTGGTGC |
| leuO | |
| leuO5′F | ACGCGTCGACGGATTGGTTCATGCTTCTTATATTTTATGGCT |
| leuO5′R | CGGGATCCTACTAAGTTGTGTTCAAACATGCTTAACTCCAC |
| leuO3′F | CGGGATCCTAGTTATTATCATTAAGTCCTGCTGCTGAAGTTG |
| leuO3′R | ATAAGAATGCGGCCGCACGCTCTTGGATGGCAGC |
| rpoS | |
| rpoS5′F | ACGCGTCGACTGTCGCTACAACCGCACC |
| rpoS5′R | GGAAGATCTCATATGCTGCTCCTACCCGTG |
| rpoS3′F | GGAAGATCTTAGCGATACTCTCGCAAACAGTCTG |
| rpoS3′R | ATAAGAATGCGGCCGCCCGTATCAAAGCCATGACGCTA |
| Reporters | |
| rovA::lacZ | |
| rlacZF | TGCTCTAGAGGGTCAATGACAAATAATAAGCCTCCAGT |
| rlacZR | CGGGATCCACCAATCGCTTTCGCCAGTTG |
| rovA::gfp | |
| pYELF | CGGGATCCTGGTTATCATGAACTAATATTTTAACCAATCGGC |
| pYESF | CGGGATCCAGGGGGATTGCATATAATAATTCCACA |
| pYEL-2R | GGAATTCACCAATCGCTTTCGCCAGTTG |
| pYPLF | CGGGATCCTGCCGCCTTCCTGCAA |
| pYPSF | CGGGATCCTTTGAAATATTGATGATTCATATCAATTTACCCAAGTC |
| pYPL-2R | GGAATTCACCAATCGCTTTCGCCAGTTG |
| Complementation clone: pLEUO | |
| pLEUOF | ACGCGTCGACGGATTGGTTCATGCTTCTTATATTTTATGGCT |
| pLEUOR | GGGGTACCTTAAGAAGGAATATTAAGCTGGCTGAGTAATTC |
| Primer extension | |
| PE1 | CTAATCGTGCTAAATCAGATCC |
| PE2 | AAAATTATGTATTTACTAAAATTACCTCTTAAGGA |
| EMSA | |
| YP1-2F | TGC CGC CTT CCT GCA A |
| YP3-4F | TTTGAAATATTGATGATTCATATCAATTTACCCAAGTC |
| YP1-4R | ACCAATCGCTTTCGCCAGTTG |
| YP2-3R | GGACAATAGCAATAAATACGGGGAA |
| YE1-2F | TGGTTATCATGAACTAATATTTTAACCAATCGGC |
| YE3F | AGGGGGATTGCATATAATAATTCCACA |
| YE4F | ATTTAAGAGACTGATGATTCATATCAATTTACCAAC |
| YE5F | TTGGATGCCAGATATCACCC |
| YE6F | ACCTAGCATAACCGCCTTAAAAATT |
| YE1-4-5-6R | ACCAATCGCTTTCGCCAGTTG |
| YE2-3R | GGACAATAGCAATAAATACGGGGAA |
| Transposon sequencing | |
| TNF | CCCATGTCAGCCGTTAAGTGT |
a
Underlining indicates restriction sites.