FIG. 7.

Reverse transcription-PCR analysis of D and V region germ line transcription in Igh WT, PDQ52^−/−, and PDQ52^−/−E_μ^−/− Rag1^−/− mice. (A) Design of the D_H gene germ line transcription reverse transcription-PCR assay. (B) D_H gene strand-specific reverse transcription-PCR of total RNA from B220⁺ BM B lymphocytes from Igh WT, PDQ52^−/−, and PDQ52^−/−E_μ^−/− Rag1^−/− mice. (C) Semiquantitative reverse transcription-PCR of D_H and V_H (V_HJ558) germ line transcription in WT and PDQ52^−/−E_μ^−/− Rag1^−/− mice. λ5 was used as a loading control.