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Mapping of an antisense transcriptional start site to the 5′ margin of the Eμ core. Reverse transcription primers were designed immediately 3′ of J4 and at the 3′ boundary of the Eμ core to detect antisense and sense transcription, respectively, across this interval. Three sets of PCR primers in the intervening region were then used to map transcription across this region. Above lanes, with (+) or without (−) reverse transcriptase (RT). DNA, genomic DNA positive control.
