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. 2007 May 21;27(15):5403–5413. doi: 10.1128/MCB.00346-07

FIG. 3.

FIG. 3.

Complementation of Yju2-depleted extracts by recombinant Yju2. (A) Splicing reactions were carried out in mock-depleted (lanes 1 and 10), Yju2-depleted (lanes 2 to 9), or NTC-depleted (lanes 11 to 13) extract with the addition of recombinant His-Yju2 (lane 3, 150 ng; lane 4, 50 ng; lane 5, 15 ng; lane 6, 5 ng; and lane 7, 2 ng) or affinity-purified NTC (lanes 8 and 12, 2 μl; lanes 9 and 13, 1 μl). (B) Western blot of mock-depleted (lane 1), NTC-depleted (lane 2), or Yju2-depleted (lane 3) extract probed with anti-Yju2, anti-HA, or anti-Ntc25 antibody. M, mock depleted; dYju2, Yju2 depleted; dNTC, NTC depleted.