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. 2007 May 21;27(15):5403–5413. doi: 10.1128/MCB.00346-07

FIG. 8.

FIG. 8.

Diagram illustrating the interaction of Yju2 with NTC, with HP, and with the spliceosome; the diagram also illustrates the function of Yju2 in the splicing reaction. Yju2 interacts with NTC and with HP in dynamic manners. Double arrowheads indicate equilibrium between the association and dissociation forms. After U1 and U4 are dissociated from the spliceosome, NTC, either in association with Yju2 or with no association, binds to activate the spliceosome. The first catalytic reaction involves an ATP-dependent step, which requires Prp2 and Spp2, and an ATP-independent step, which requires HP and Yju2. The second catalytic reaction also involves an ATP-dependent step, which requires Prp17 and Prp16, and an ATP-independent step, which requires Slu7, Prp18, and Prp22. The requirement for ATP in each step is indicated. Yju2 is destabilized from the spliceosome after the reaction is complete, but its association with the spliceosome after the Prp16 step remains undetermined and is indicated by a question mark. A change in the color of the spliceosome represents a structural change of the spliceosome during each transition.