FIG. 5.

G₂/M cell cycle arrest induced by SC35 deficiency in MEFs. (A) Analysis of proliferating cells by BrdU labeling before and after SC35 depletion (5 days after Dox addition to the media). (B) FACS analysis of the cell cycle in wt and SC35-depleted MEFs (+Dox), showing a reduction in the number of S-phase cells and a dramatic increase in the number of G₂/M-phase cells. (C) Analysis of M-phase cells on the basis of staining for the mitotic phosphorylated histone H3 (p-Histone H3) marker (+Dox, 4 days). (D and E) Experimental design (D) and time course FACS analyses (E) of cell cycle progression of SC35-deficient MEFs (+Dox). (F) Immunocytochemical analyses of SC35-depleted cells (5 days after Dox treatment) by staining with an antibody against phosphorylated γH2AX to detect induced foci accumulated on broken DNA. PI, propidium iodide.