FIG. 1.
Effect of HuR knockdown on cell growth. (A) Effect of HuR depletion on cell adhesion. HeLa cells transfected with siCtr or siHuR were seeded on six-well plates with gelatin (upper panels) or fibronectin (lower panels). Adherent cells were also stained with DAPI (data not shown). (B) The nuclei were counted by fluorescence microscopy. Relative numbers of cells are shown as means ± standard deviations for three experiments. For each experiment, pictures of 10 fields (equal size) were taken and the cells were counted. (C) Doubling time of HeLa cells knocked down for HuR. HeLa cells (2 × 105) were seeded on six-well plates and then treated with siRNAs as described for panel A. Cells were counted every day for 5 days. Data represent the means ± standard deviations for two independent experiments, each performed in triplicate.