FIG. 3.

[SP14⁺] contains large aggregates which are unaffected by prion stabilization. (A) Protein aggregates and monomeric Sup35p from wild-type [PSI⁺] (strong [s] and weak [w]) and [SP5⁺] and [SP14⁺] (original isolate, weak and strong) strains were separated by SDD-AGE and detected by Western blotting. (B) Wild-type [PSI⁺] and [SP14⁺] cells with and without the overexpression (OE) of Hsp104p plated on YPD. (C) Protein aggregates and soluble protein (monomer) from lysates of [SP14⁺] cells with (+) or without (−) Hsp104p overexpression were separated by SDD-AGE and detected by Western blotting.