TABLE 1.
Summary of subtle wobble splicing that utilizes two successive 3′ splice sites
| Tandem motifa | AG selection (%)b
|
|
|---|---|---|
| Proximal position | Distal position | |
| AAG AAG | 8.8 | 91.2 |
| AAG TAG | 3.0 | 97.0 |
| AAG CAG | 1.5 | 98.5 |
| AAG GAG | 81.2 | 18.8 |
| TAG AAG | 45.1 | 54.9 |
| TAG TAG | 20.2 | 79.8 |
| TAG CAG | 7.9 | 92.1 |
| TAG GAG | 96.8 | 3.2 |
| CAG AAG | 48.3 | 51.7 |
| CAG TAG | 26.0 | 74.0 |
| CAG CAG | 12.1 | 87.9 |
| CAG GAG | 96.9 | 3.1 |
| GAG AAG | 0 | 100 |
| GAG TAG | 0 | 100 |
| GAG CAG | 0 | 100 |
| GAG GAG | 1.9 | 98.1 |
a
The 5′ tandem splice site mutation mini-genes (H-H-H5′ site mutant) in which the “N” position was replaced with A, T, C, or G were transfected into human AZ521 cells, and the wobble splicing patterns were analyzed by capillary electrophoresis.
b
The ratio of the distal or proximal AG selection was determined by dividing the individual peak area by the total area.