TABLE 3.
Accession no.a | EST countb
|
Exon conservation (%)c | Intron conservation (%)d | Conservation in the 60-bp region preceding NAGNAG (%)e | |
---|---|---|---|---|---|
Human | Mouse | ||||
NP_056371 (SIPA1L1)* | 33/14 | 2/27 | 92 | 22 | 80 |
NP_055948 (MAPK8IP3)* | 1/4 | 4/3 | 90 | 1 | 56 |
NP_055041 (RAGE)* | 7/9 | 9/0 | 88 | 5 | 28 |
NP_060920 (ARID1A)* | 14/14 | 0/18 | 93 | 46 | 49 |
NP_037395 (BRD7)* | 8/19 | 1/36 | 82 | 27 | 70 |
Avg | 89 | 20.2 | 56.6 | ||
NP_003196 (TCF12) | 12/4 | 31/2 | 91 | 13 | 61 |
NP_003063 (SMARCA4) | 77/27 | 39/10 | 92 | 1 | 100 |
NP_003893 (FUBP1) | 48/47 | 50/48 | 92 | 40 | 70 |
NP_003361 (VASP) | 27/3 | 20/2 | 86 | 44 | 71 |
NP_055491 (PUM1) | 46/3 | 48/1 | 95 | 5 | 98 |
Avg | 91.2 | 20.6 | 80 |
*, statistical differences were found between species-specific splicing patterns of human and mouse.
The number of insertion and deletion isoforms were counted by NCBI human and mouse EST database screening.
Conservation of exon sequences flanking the wobble splicing junction is indicated. Human/mouse alignments were analyzed, and conservation scores were calculated by ClustalW software.
Conservation of intron sequences upstream of the NAGNAG tandem motif is indicated.
Sequence conservation was observed for the 60-bp region upstream of the splice sites, including the sequence of the 3′ tandem splice site.