FIG. 2.

Vacuolar accumulation of Trk1 is not caused by changes in osmotic pressure during potassium starvation. (A) Western analysis of the insoluble proteins isolated from the indicated strains transformed with either an empty or a HAL5-containing centromeric plasmid incubated for 2 h in unsupplemented minimal medium (−) or medium supplemented with 0.2 M KCl or 0.2 M NaCl. The amount of Trk1 was analyzed using the anti-HA antibody (top panel), and the amount of protein present in each sample is shown in the Ponceau S (PonS)-stained filter (bottom panel). (B) Confocal microscopy images of Trk1-GFP localization in the hal4 hal5 mutant under the same experimental conditions, including an additional control using an osmotically equivalent amount of sorbitol (0.3 M).