FIG. 5.

Subcellular localization of the Gap1 permease and the integral membrane protein, Sur7. (A) The subcellular localization of Gap1-GFP was analyzed in the indicated strains grown to mid-log phase in potassium-supplemented (50 mM) minimal medium with proline as the sole nitrogen source. Representative micrographs are shown. WT, wild type. (B) The subcellular localization of the eisosomal-associated integral membrane protein Sur7 in the hal4 hal5 mutant was analyzed by confocal microscopy after 2 h of incubation in minimal medium (without KCl). Identical Sur7-GFP patterns were observed in the wild-type strain and in the hal4 hal5 strain grown in the presence of KCl (data not shown).