FIG. 9.
IRS-1 tyrosine phosphorylation in wild-type (wt) and Grb10Δ2-4m/p (ko) mice. (A) Tissue lysates from both control (−insulin) and insulin-stimulated (+insulin) animals were Western blotted with antibodies specific for Y612-phosphorylated IRS-1 and for total IRS-1. Blots of quadriceps muscle lysates are shown as an example. (B to D) Absolute levels of insulin-stimulated IRS-1 tyrosine phosphorylation (left panels) and relative levels of tyrosine-phosphorylated IRS-1 (that have been corrected for total IRS-1) (right panels) in quadriceps muscle (B), tibialis muscle (C), and WAT (D) are shown. All results are expressed as means ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001 [Student's t test]). AU, arbitrary units.