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Expression of chondrocyte differentiation markers in wild-type and Hmgb1−/− tibia. (A and B) Indian hedgehog (Ihh) is comparable between wild-type and Hmgb1−/− embryos at E14.5. (C to F) Col10a1 appears in the region of hypertrophic chondrocytes at E14.5 (C and D) and then declines in the most mature hypertrophic chondrocytes at the center of hypertrophic zones at E15.5 in both groups without an apparent difference between wild-type and mutant embryos (E and F). Scale bars, 200 μm.
