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. 2007 Jun 4;27(16):5664–5672. doi: 10.1128/MCB.00602-07

FIG. 5.

FIG. 5.

Expression of the human β-like globin genes in the HS1/λ/Bepsi TgM. (A) Total RNA was prepared from the yolk sacs of more than two embryos (9.5 dpc) from two independent litters of each mutant line. Expression of human Bepsi (hBepsi)- and human γ (hγ)-globin compared to endogenous mouse α (mα)-globin genes was separately analyzed by semiquantitative RT-PCR. λ, HS1/λ/Bepsi; loxP, HS1/loxP/Bepsi. (B) HS1/λ/Bepsi TgM (line 920) was bred with the EKLF-null mouse to analyze β-like globin gene expression in the presence (EKLF+/+) or absence (EKLF−/−) of endogenous EKLF activity. Total RNA was prepared from the yolk sacs of more than two embryos (9.5 dpc) from two independent litters of the mutant line. Expression of hBepsi- and hγ-globin compared to endogenous mα-globin genes was separately analyzed by semiquantitative RT-PCR. See the legend to Fig. 3 for details.