TABLE 1.
Hematopoietic profile of mice carrying mutations in Pgm3a
| Parameter | Value ± SE
|
||
|---|---|---|---|
| Pgm3+/+ | Pgm3mld1/mld1 | Pgm3mld1/gt | |
| Peripheral blood | |||
| Red blood cells (109/ml) | 10.4 ± 0.5 | 8.0 ± 0.62 | 6.5 ± 2.0 |
| Hematocrit (%) | 53.2 ± 2.4 | 45.8 ± 3.0 | 37.9 ± 10.9 |
| Platelets (106/ml) | 1,159 ± 176 | 620 ± 128 | 858 ± 312 |
| White blood cells (106/ml) | 9.2 ± 1.9 | 3.7 ± 1.3 | 4.8 ± 2.1 |
| Lymphocytes (106/ml) | 8.2 ± 1.8 | 3.5 ± 1.7 | 3.5 ± 1.7 |
| Megakaryocytes (per 100 fields at ×200 magnification) | |||
| Bone marrow | 116 ± 44 | 107 ± 37 | 115 ± 29 |
| Spleen | 2 ± 1 | 33 ± 11 | 39 ± 22 |
| Colony-forming progenitor cells | |||
| Bone marrow (per 2.5 × 104 cells) | |||
| BLAST | 10.3 ± 6.1 | 10 ± 2.8 | ND |
| Granulocyte | 19.5 ± 6.6 | 29.5 ± 2.1 | ND |
| Granulocyte/macrophage | 15.3 ± 4.8 | 15.5 ± 2.1 | ND |
| Macrophage | 13.3 ± 4.9 | 21 ± 11.3 | ND |
| Eosinophil | 0.8 ± 1.0 | 0.5 ± 0.7 | ND |
| Megakaryocyte | 27.8 ± 9.1 | 7 ± 1.4 | ND |
| CFU-E | 30.6 ± 9.4 | 85.9 ± 30.6 | ND |
| BFU-E | 6.4 ± 18.5 | 18.5 ± 5.6 | ND |
| Spleen (per 5.0 × 104 cells) | |||
| BLAST | 0.4 ± 0.5 | 12 ± 0 | ND |
| Granulocyte | 0.6 ± 1.0 | 15 ± 5.7 | ND |
| Granulocyte/macrophage | 0.1 ± 0.3 | 10 ± 8.5 | ND |
| Macrophage | 0.8 ± 1.0 | 12 ± 2.8 | ND |
| Eosinophil | 0 | 0.5 ± 0.7 | ND |
| Megakaryocyte | 4.8 ± 2.2 | 12.5 ± 0.7 | ND |
| CFU-E | 2.5 ± 1.3 | 47.3 ± 7.3 | ND |
| BFU-E | 2.8 ± 1.1 | 20.3 ± 10.3 | ND |
a
For peripheral blood data, values represent the means ± standard errors for 15 to 52 mice; for all other data, values represent the means ± standard errors for two to four mice. ND, not determined.