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Cdk8 is required at E2.5. (A) Embryos isolated at E2.5 of Cdk8+/− intercrosses were genotyped using nested PCR. E2.5 is the last stage of embryogenesis where Cdk8−/− embryos can readily be isolated. Molecular size markers are indicated on the right. (B) Phase-contrast photomicrographs of Cdk8+/+ and Cdk8−/− embryos at E2.5. Black and white arrowheads indicate fragmented blastomeres.
