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. 2007 Jun 25;27(17):6195–6208. doi: 10.1128/MCB.02065-06

TABLE 2.

Effects of t-BHQ on the representative antioxidant enzyme gene expression

Gene Gene product nameb Relative change in gene expression in cells with indicated genotypea
RK−/− Gα12−/− Gα13−/−
Antioxidant enzyme genes
    Hmox1 Heme oxygenase (decycling) 1 9.90 13.11 11.59*
    Txnrd1 Thioredoxin reductase 1 1.50** 5.97*† 2.19*
    Gsr Glutathione reductase 1 1.16 2.85*† 2.13*
    Cat Catalase 1.18 2.20* 1.38**
    Prdx1 Peroxiredoxin 1 1.27 NA 1.56
Xenobiotic metabolism genes
    Nqo1 NAD(P)H dehydrogenase quinone 1 4.32 16.01** 3.13**
    Gclm Glutamate-cysteine ligase modifier subunit 2.30* 10.39**‡ 2.22**
    Gclc Glutamate-cysteine ligase catalytic subunit 2.16* 9.72**‡ 3.27**†
    Gsta4 GlutathioneS-transferase alpha 4 3.64* 7.27**† 3.69**
    Gsta3 Glutathione S-transferase alpha 3 NA 5.51 1.13
    Gsta2 GlutathioneS-transferase alpha 2 (Yc2) 1.10 3.43*† 2.71*‡
    Gstp1 Glutathione S-transferase pi 1 1.37* 2.40 1.43*
    Gss Glutathione synthetase 1.39 2.31 1.37*
    Gsto1 Glutathione S-transferase omega 1 1.87** 1.84 1.66*
    Gstm3 Glutathione S-transferase mu 3 1.51 1.82* 1.88**
    Gstm1 GlutathioneS-transferase mu 1c 2.60** 1.54† 3.00**
    UGT1A6 UDP glycosyltransferase 1 family A6d 2.98** 1.40† 2.55**†
    Nqo2 NAD(P)H dehydrogenase quinone 2 1.62* 1.19 1.21
    Mgst1 Microsomal glutathione S-transferase 1 2.07* 1.15 1.84*
    Gstm4 Glutathione S-transferase mu 4 1.09 1.14 0.94†
    Gstk1 Glutathione S-transferase kappa 1 0.91 1.09 0.86
    Gstm5 Glutathione S-transferase mu 5 1.03 0.93 1.03
    Mgst3 Microsomal glutathione S-transferase 3 1.03 0.92 1.21
    Gstm6 Glutathione S-transferase mu 6 1.43 0.84 1.29*
    Gstz1 Glutathione S-transferase zeta 1 0.95 0.82 0.87
    Gstm2 Glutathione S-transferase mu 2 1.52 0.66 1.25*
    Gstt3 Glutathione S-transferase theta 3 1.33 0.65 NA
    Gstt1 Glutathione S-transferase theta 1 0.78** 0.50 0.81
a

Each value indicates the relative change after t-BHQ treatment (30 μM, 12 h). Asterisks indicate significant difference from value for vehicle treatment (*, P < 0.05; **, P < 0.01). Daggers indicate significant difference from RK−/− values (†, P < 0.05; ‡, P < 0.01). NA, data not available.

b

Genes for products in bold are Nrf2 dependent.

c

Basal mRNA content was 2.4-fold greater in Gα12−/− cells than in control cells.

d

Basal mRNA content was 42-fold greater in Gα12−/− cells than in control cells.