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TRAF6 is not the only E3 ubiquitin ligase responsible for NOD2/RIP2-induced NEMO ubiquitination. (A) TRAF6 expression was inhibited by siRNA (second blot from bottom) using two siRNAs designed to be unique to TRAF6, and RIP2-induced NEMO ubiquitination assays were performed as described previously (1). (B) TRAF6 or RIP2 expression was inhibited by siRNA, and NOD2-induced NF-κB activation was assayed (300 ng NOD2, 200 ng NF-κB reporter, 200 ng Renilla luciferase).
